A novel label-free NIR aptasensor based on triphenylmethane dyes for rapid detection of salicylic acid

Abstract

Salicylic acid (SA) is a critical phytohormone involved in plant growth, development, and defense responses, making its precise quantification essential for both agricultural management and environmental monitoring. Here, we report a novel label-free near-infrared aptasensor (NIRApt) for the rapid and sensitive detection of SA, utilizing a rationally selected triphenylmethane (TPM) dye. Through systematic screening, ethyl violet (EV) was identified as the optimal fluorophore, showing pronounced fluorescence enhancement upon binding to a SA-specific aptamer. The sensing mechanism relies on target-induced competitive displacement, in which SA binding triggers the release of EV from the aptamer complex, resulting in a marked quenching of the fluorescence signal. The developed NIRApt exhibits high specificity and sensitivity, with a detection limit of 0.63 μM and a broad linear range of 0–30 μM. Remarkably, it demonstrates an ultrafast response within one minute, highlighting its efficiency for rapid quantitative detection. Its practicality was validated in spiked cherry tomato samples, achieving high detection accuracy. This platform offers key advantages including label-free operation, low background signal, minimal sample preparation and rapid response, demonstrating strong potential for food safety analysis and environmental monitoring of SA.

Supplementary files

Article information

Article type
Paper
Submitted
18 Jun 2025
Accepted
16 Aug 2025
First published
20 Aug 2025

Anal. Methods, 2025, Accepted Manuscript

A novel label-free NIR aptasensor based on triphenylmethane dyes for rapid detection of salicylic acid

L. Bu, X. Li, J. Hu, M. Liang, L. Li and T. P. Loh, Anal. Methods, 2025, Accepted Manuscript , DOI: 10.1039/D5AY01023C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements