Monitoring Nucleoside Metabolism in Living Cells with a Nucleobase Analogue via Fluorescence Lifetime Imaging
Abstract
To overcome challenges in fluorescence labelling of RNA inside living cells we have recently introduced a direct approach using the fluorescent nucleobase analogue 2CNqA. Here we demonstrate its potential for use in fluorescence lifetime imaging (FLIM) to investigate nucleoside metabolism and for metabolic RNA labelling.
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