Formation dynamics of patchy/Janus DNA condensates in monodisperse giant vesicles generated using microfluidics
Abstract
Herein, we demonstrate the one-step formation of uniform patchy or Janus-type DNA condensates within monodisperse giant vesicles. This process was achieved by encapsulating DNA building blocks in monodisperse giant vesicles fabricated using a microfluidic device and then subjecting them to gradual concentration by shrinking the vesicles using osmotic action. The concentration of the linker DNA motif, which connects two immiscible condensates with orthogonal DNA nanostructures, was identified as a crucial parameter determining the morphology of patchy/Janus DNA condensates. Furthermore, the formation of condensates within uniformly sized giant vesicles allowed the production of patchy DNA condensates with a consistent morphology. Our approach is expected to offer a promising method for homogeneous production of artificial cells with complex functionalities.
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